Bio-One Colorado

    Ringside Development Company d/b/a Bio-One Colorado, Inc. · reviewed 2026 disclosure data

    Researching Bio-One Colorado franchise opportunities? Every figure below is cited to the FDD.

    Reviewed sources

    Bio-One is a crime and trauma scene cleaning franchise that provides biohazard remediation and cleanup services. Its services include suicide and homicide cleanup, hoarding cleanup, blood spill cleanup, decomposition cleanup, infectious disease disinfection, meth lab cleanup, medical waste disposal, sewage backup cleanup, odor removal, rodent-dropping removal, and homeless encampment cleanup.

    Headline figures

    Quick facts

    How much does it cost to open a Bio-One Colorado franchise?
    The Bio-One Colorado Franchise Disclosure Document reports an estimated initial investment of $134,645 (2026 FDD, Item 7, PDF p. 18). This is the franchisor's disclosed estimate, not a quote. Details
    What is the Bio-One Colorado initial franchise fee?
    The disclosed initial franchise fee for a standard single unit is $60,000 (2026 FDD, Item 5, PDF p. 12). Other fee options (for example multi-unit or conversion) are listed separately in the report. Details
    What ongoing fees does Bio-One Colorado charge?
    The disclosed ongoing royalty is the greater of applicable Minimum Royalty or 7.5% of Gross Sales (2026 FDD, Item 6, PDF p. 13). The brand or advertising fund contribution is the greater of $200 or 2% of Gross Sales; may increase to no more than 3% (2026 FDD, Item 6, PDF p. 13). Other recurring fees are listed in Item 6. Details
    Does Bio-One Colorado disclose financial performance (Item 19)?
    Yes. The Bio-One Colorado FDD includes an Item 19 financial performance representation (2026 FDD, Item 19, PDF p. 37). Figures apply only to the locations and period each one covers. Details
    How many Bio-One Colorado locations are there?
    The FDD reports 147 outlets at the end of 2025 (2026 FDD, Item 20, PDF p. 39). Existing locations do not indicate territory availability. Details

    From reviewed FDD figures only. Not a recommendation or projection.

    What the disclosures show

    1. 1Opening costs are estimated at $134,645 in the 2026 FDD (Item 7); several initial fee options apply.
    2. 2Item 19 covers 2 separate groups: revenue for 110 franchised locations; adjusted operating results for 61 franchised franchisees. They are not combined.
    3. 3Outlets declined by 5 in 2025, to 147 (Systemwide, as labeled in the FDD, Item 20).

    From reviewed FDD figures only. Not a recommendation or projection.

    Costs · Items 5–7

    What does it cost to open?

    Estimated opening costs and the fees payable to the franchisor.

    Startup Investment — Item 7

    Total estimated initial investment

    2026 FDD · Item 7 · PDF p. 18

    Standard single-unit franchise fee

    2026 FDD · Item 5 · PDF p. 12
    Discounted and multi-unit options are separate; see all fees.

    Ongoing Fees — Item 6

    Selected recurring charges; payment bases and conditions remain attached to each fee.

    RoyaltyMain ongoing fee · The minimum royalty amount is not stated in the provided fee row or source excerpt.

    26 reported fees, each with its payment basis, are listed below; they haven't been sorted into recurring, initial and occasional groups yet.

    Financial Performance — Item 19

    What results are disclosed—and for which locations?

    Two separate populations, not a revenue-and-profit comparison. Revenue is not owner income.

    Franchised locations

    Revenue · 110 locations

    Who is counted
    Franchisees who have reported 12 months of revenue or more
    Period
    2025
    Excluded
    Those who were not in operation for each of the 12 months in 2025; no Franchisees have been excluded, unless they did not report

    Franchised locations

    Adjusted operating results · 61 locations

    Adjusted company-owned results: the franchisor adjusted these centers' expenses to estimate franchisee costs. They are not observed franchisee results or owner take-home income.

    Who is counted
    all 61 active franchisees in good standing who supplied 2025 P&L Reports, operating in 75 locations
    Period
    2025
    Excluded
    none have been excluded save for those who had not been operational for such time or those for whom data was not received
    Method
    The Adjusted EBITDA makes final adjustments to assure that the applicable royalty rate and any other fees paid to Bio-One as the franchisor have been matched to those you will incur under your franchise agreement, even if prior versions may be historically inconsistent on some terms relating to such fees.

    These groups are never combined. Adjusted company-owned operating results cannot be divided by franchised revenue to calculate a franchisee profit margin.

    Outlet Changes — Item 20

    How has the outlet count changed?

    Systemwide, as labeled in the FDD · counts as of year end of each year.

    2026 FDD · Item 20 · PDF p. 39

    Net +1 outlets, 2023–2025

    0146202315220241472025+6−5

    Year-end counts reported in Item 20. The scale starts at zero, so the line looks nearly flat; the labels between the dots show each year's change. Openings and closures for 2025 haven't been pulled from the Item 20 tables yet, so no turnover or churn is shown.

    Questions to investigate

    Prompts based on what this brand's reviewed disclosures do and don't show — not conclusions. Join free as a member to save questions to your research.

    For the franchisor

    • Which line items in the 2026 Item 7 estimate ($134,645) vary most by market, and why?

    • Which initial fee option would apply to my situation, and what conditions or discounts affect it?

    • Which locations are included in the Item 19 figures, and which were excluded?

    • Reported units decreased from 152 to 147 in 2025. What drove the change?

    • Is a territory available in my area, and how is it defined in my agreement?

    For current and former owners

    • How did your actual startup costs and timeline compare with the Item 7 estimate?

    • How many hours a week do you work in the business, and in what role?

    • How do your results compare with the Item 19 figures, and what explains the difference?

    • What support from the franchisor has been most and least useful?

    For your advisors

    • Ask a franchise attorney to review the territory, renewal, transfer and termination terms.

    • Ask an attorney what the Item 3 litigation disclosures mean for a new franchisee.

    • Ask an accountant to build a cash-flow plan using your own assumptions, not the franchisor's.

    Sources & vintage history

    Every displayed fact is retained with its disclosure vintage, item, page, and reviewed status. Prior vintages remain on file rather than being overwritten.

    Citations (2026 FDD)

    1. [1]Estimated initial investment — Item 7 · PDF p. 18
    2. [2]Standard initial franchise fee — Item 5 · PDF p. 12
    3. [3]Item 19 — Franchised (110 locations) — revenue · PDF p. 37
    4. [4]Item 19 — Franchised (61 franchisees) — adjusted operating results · PDF p. 37
    5. [5]Outlet counts — Item 20, Table 1 · PDF p. 39

    "PDF p." is the page in the PDF file; "printed p." is the number printed on the FDD page. Source document: FDD

    Territory & operations

    112 addresses in Franopolis's imported location list. Coverage may differ from Item 20 outlet counts.

    These are contract disclosures, not confirmation that any territory is currently available.

    Franchisor financial statements

    No verified franchisor financial statement figures have been reviewed yet.

    SBA-backed loans to franchisees

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    Source: U.S. Small Business Administration 7(a) & 504 FOIA data. Loan volume is not a measure of franchisee success.

    At a glance

    Business history

    Owner participation

    Territory language

    Litigation disclosure

    Bankruptcy disclosure

    Agreement term

    Independent analysis of franchisor-provided data. Not investment advice. Franopolis does not distribute FDDs. Last reviewed 10/5/2026.