Bio-One Colorado franchise cost 2026: $135K–$135K
The 2026 Bio-One Colorado FDD puts the estimated initial investment at $134,645 to $134,645 (Item 7, p. 18). It lists a $1,595 initial franchise fee, a royalty of greater of applicable Minimum Royalty or 7.5% of Gross Sales and a marketing fund contribution of greater of $200 or 2% of Gross Sales; may increase to no more than 3%.
Quick answer. The 2026 Bio-One Colorado FDD puts the estimated initial investment at $134,645 to $134,645 (Item 7, p. 18). It lists a $60,000 standard initial franchise fee, a royalty of greater of applicable Minimum Royalty or 7.5% of Gross Sales and a marketing fund contribution of greater of $200 or 2% of Gross Sales; may increase to no more than 3%.
Key takeaways
- Estimated initial investment: $134,645 to $134,645 (Item 7, p. 18).
- The largest single line is "IFF" at $60,000 (Item 7, p. 17).
- Initial franchise fee (standard single unit): $60,000 (Item 5, p. 12). Other fee variants apply; see the fee table below.
- Royalty: greater of applicable Minimum Royalty or 7.5% of Gross Sales (Item 6, p. 13).
- Marketing fund: greater of $200 or 2% of Gross Sales; may increase to no more than 3% (Item 6, p. 13).
- Outlets on file in Item 20 went from 144 at the start of 2023 to 147 at the end of 2025 (Item 20).
Estimated initial investment (Item 7)
Item 7 of the 2026 FDD lists the following ranges. These are the franchisor's estimates, not quotes for any specific location.
| Line | Low | High |
|---|---|---|
| IFF | $60,000 | $60,000 |
| QSP Package | $39,500 | $39,500 |
| Technology Startup Fee | $1,595 | $1,595 |
| Annual Conference Registration Deposit | $2,000 | $2,000 |
| Furniture, Fixtures, and Equipment | $0 | $2,000 |
| Initial Training Expenses | $0 | $500 |
| OSHA Training Fee | $2,000 | $2,000 |
| OSHA Training Expenses | $0 | $500 |
| Business Vehicle | $0 | $0 |
| Business Vehicle Tax, Title and License | $0 | $0 |
| Certifications | $2,500 | $3,500 |
| Insurance and Professional Services | $1,250 | $4,000 |
| Additional Funds - 3 months | $15,000 | $15,000 |
| Business Vehicle Insurance | $1,000 | $1,000 |
| US Local Marketing | $9,000 | $9,000 |
| Rent and Rental Improvements | $0 | $0 |
| Computer Hardware, Software and Other Electronics | $800 | $800 |
| TOTAL | $134,645 | $134,645 |
Fees (Items 5 and 6)
| Fee | Amount | Basis |
|---|---|---|
| Technology Startup Fee | $1,595 | — |
| OSHA Training Fee | $2,000 | — |
| Annual Conference Registration Deposit | $2,000 | — |
| Initial franchise fee (IFF) | $60,000 | — |
| QSP Package | $39,500 | — |
| Successor Franchise Fee | $10,000 | per Successor Franchise Rights award |
| QSP Consumables Replacement Inventory | $100 | per month |
| Client Service Fee | $0 | currently $0; all costs incurred may also be charged |
| Late Fee | $150 | per month per late fee |
| Report Late Fee | $50 | per day |
| Audit Fees | $750 | plus costs and expenses |
| Temporary Management Fee | $1,500 | per day plus expenses |
| Marketing Fee | $200 | greater of $200 or 2% of Gross Sales; may increase to no more than 3% |
| Local Advertising Fee | $3,000 | greater of $3,000 or 5% of Gross Sales |
| Regional Advertising Cooperative Fee | $0 | Currently $0; future specified part of Local Advertising Fee |
| Accounting Software Fees | $0 | per month |
| Additional Training Fee | $1,500 | plus travel, room and board if applicable |
| OSHA Training Fee | $2,000 | plus travel, room and board |
| Mandatory Non-Attendance Fee | $1,500 | per occurrence; may change upon notice |
| QSP Package Maintenance and Renewal Fee | $10,000 | no more often than once every five years |
| Transfer Fee | $15,000 | per permitted transfer |
| Approval of New or Substitute Vendor | $0 | currently $0; may be charged in the future |
| Technology Maintenance Fee | $650 | per month |
| Substitute or New Designated Manager Training Fee | $1,500 | per person, plus attendance expenses |
| Default Interest | $15 | per annum, compounded monthly |
| Royalty | $8 | greater of applicable Minimum Royalty or 7.5% of Gross Sales |
Financial performance (Item 19)
These figures are reported by the franchisor. Read the population, period and basis before comparing them with anything else, and remember that gross sales are not profit.
| Metric | Statistic | Value | Period | Who is counted |
|---|---|---|---|---|
| Franchisee average revenues - Tier 1 | reported | $533,389 | 2025 | Not stated in our record |
| Franchisee average revenues - Tier 3 | reported | $148,781 | 2025 | Not stated in our record |
| Franchisee average revenues - Tier 4 | reported | $5,280 | 2025 | Not stated in our record |
| Franchisee average revenues - Tier 2 | reported | $314,948 | 2025 | Not stated in our record |
| Adjusted EBITDA | reported | $-167 percent of revenue | 2025 | Not stated in our record |
Outlets (Item 20)
| Year | Outlet type | Start of year | End of year |
|---|---|---|---|
| 2023 | company owned | 0 | 0 |
| 2023 | franchisee owned | 144 | 146 |
| 2024 | company owned | 0 | 0 |
| 2024 | franchisee owned | 146 | 152 |
| 2025 | company owned | 0 | 3 |
| 2025 | franchisee owned | 152 | 144 |
Calculated: net change in outlets 2023–2025 = end of 2025 (147) − start of 2023 (144) = +3. Outlet counts describe the size of the system, not how individual owners performed.
Questions to bring to your own research
- Which Item 7 lines depend most on your local rent, buildout and labor costs?
- Which fees in Item 6 are fixed, and which rise with sales or at renewal?
- Which units are included in the Item 19 figures, and which are excluded?
- What do current and former franchisees say about the time it took to open and the costs they actually paid?
Sources and method
Every figure above comes from the Bio-One Colorado 2026 Franchise Disclosure Document and was checked against the document by a reviewer before it appeared on Franopolis. Figures marked "Calculated" are Franopolis arithmetic, with the formula shown. This entry is not investment, legal or tax advice, and it does not rank or recommend any franchise.
See the full Bio-One Colorado research profile, model your own numbers in the Investment Planner, or read the territory disclosures.